High-performance liquid chromatography of nalbuphine, butorphanol and morphine in blood and brain microdialysate samples: application to pharmacokinetic/pharmacodynamic studies in rats

Publication Type:

Journal Article

Authors:

Groenendaal, D.; Blom-Roosemalen, M.C.; Danhof, M.; Lange, E.C.

Source:

J Chromatogr B Analyt Technol Biomed Life Sci, Volume 822, Number 1-2, p.230-7 (2005)

ISBN:

1570-0232 (Print)

DOI Name (links to online publication)

10.1016/j.jchromb.2005.06.008

Keywords:

Animals; Butorphanol/*analysis/blood; Chromatography; High Pressure Liquid/*methods; Male; Microdialysis; Morphine/*analysis/blood; Nalbuphine/*analysis/blood; Narcotics/*pharmacokinetics; Rats; Rats; Wistar; Reproducibility of Results

Abstract:

A rapid and sensitive assay for quantification of nalbuphine, butorphanol and morphine in blood (50 microL) and brain microdialysate ( approximately 40 microL) samples was developed. Blood samples were extracted with ethyl acetate. Analysis was performed with high-performance liquid chromatography (HPLC) coupled to an electrochemical detector. The mobile phase was a mixture of 0.1 M sodium phosphate buffer, methanol and octane-sulfonic acid with ratio and pH depending on compound and matrix. The limits of quantification in blood samples were 25, 50 and 25 ng/mL for nalbuphine, butorphanol and morphine, respectively and 0.5 ng/mL for morphine in microdialysate samples. Based on sample volume, sensitivity and reproducibility, these assays are particularly suitable for pharmacokinetic/pharmacodynamic studies in rodents.